FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_rad27-asynch.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-asynch.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11127878
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG1513491.360088599102183TruSeq Adapter, Index 18 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1031750.040.89575666
CTTGAAA1047100.040.7768667
ACGTGTG328450.040.7273831
TGCTTGA1020450.040.11002765
CTGCTTG1019550.039.94921564
TGAAAAA1166050.039.5257769
GAAAAAA1199450.039.0990370
TTGAAAA1117150.038.6180568
TCTGCTT1072750.038.20519363
CTTCTGC1092150.038.16410461
TTCTGCT1069300.037.657362
GTCTTCT1132150.036.2703459
TCTTCTG1141700.035.99389360
ATGCCGG115600.034.33451570
GACGTGT133200.033.8087541
CCGTCTT1264900.033.7062457
CGTCTTC1260000.033.41230458
AAAAAGG307950.032.7786370
GCCGTCT1303500.032.43139356
GCGTGAA59950.032.1697766
Produced by FastQC (version 0.11.5)