FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264799
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4553702.983137871648359TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG438020.28694776786775905TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG545500.053.5382470
CTGCTTG670350.050.86757757
TGCTTGA699800.048.87565658
GCCGTCT715750.048.6610449
CCGTCTT724050.048.29658550
TGCCGTC737200.048.2569248
GCTTGAA727450.047.94157459
ATGCCGT751700.047.5875947
TATGCCG765850.047.0855346
CGTCTTC751350.046.39718651
TCTGCTT740550.046.3622956
CTTCTGC749250.046.16934654
GTATGCC795450.045.491545
TATCTCG771300.044.79878639
GTCTTCT770550.044.64605752
CGTATGC812250.044.3308344
TTCTGCT762600.044.21882655
TCTTCTG779150.044.16761453
CTCGTAT792900.044.0397442
CTTGAAA799700.043.98268560
Produced by FastQC (version 0.11.5)