FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12514684
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2203551.760771586402022TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG167600.13392267835128718TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG363100.050.31892857
TGCTTGA385300.047.5917158
AAAAGGG305000.047.4282570
GCCGTCT391750.046.82581749
TGCCGTC396750.046.6240548
CCGTCTT394900.046.5586650
GCTTGAA399850.046.0787559
ATGCCGT407400.045.4907847
TATGCCG409200.045.34090846
CGTCTTC426100.043.22345751
TCTGCTT424750.043.16341456
GTATGCC431400.043.09689345
CTTCTGC435600.042.2011254
CGTATGC444950.041.84723344
CTCGTAT426650.041.5338842
GTCTTCT444400.041.3252552
TCGTATG452050.041.1357743
AATCTCG417400.041.1202439
GACCAAT435450.041.0867235
ACTGACC452650.041.0401232
Produced by FastQC (version 0.11.5)