FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_dna2-exoI-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_dna2-exoI-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15158613
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG312300.20602148758596844TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG208250.021.88278464
ACGTGTG86750.020.8284051
TGCTTGA232100.019.81538865
GCTTGAA249800.019.21012766
GACGTGT61350.018.6071051
CGTGTGC111500.018.3092041
ATGCCGG38850.017.74821370
CCGTCTT280000.017.12550557
CCGATCT253800.017.0644476
GCCGTCT283650.016.99145756
TCCGATC258150.016.8447135
TCTGCTT285950.016.291263
TGCCGTC301200.016.21106755
CTTCCGA273450.016.1198313
CTTGAAA309100.015.92099167
ATGCCGT312000.015.8629154
CTTCTGC299000.015.743937561
TCGGCCG26550.015.68593225
CGATCTA96450.015.6750157
GTGTGCT131350.015.6470392
Produced by FastQC (version 0.11.5)