FastQCFastQC Report
Sat 26 May 2018
H3HF7AFXY_n01_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3HF7AFXY_n01_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12230949
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2078651.6995001777866952TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG188840.1543952149583814TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG322750.050.3077857
TGCCGTC346600.048.3615848
GCCGTCT340550.048.264349
CCGTCTT344750.048.00150
AAAAGGG284700.047.90775370
TATGCCG356550.047.5613346
ATGCCGT355350.047.43726747
TGCTTGA344600.047.40231758
CATCTCG343250.046.08642239
GCTTGAA363450.045.68536459
GTATGCC376750.045.1413245
GCATCTC352400.044.97881338
AGGCATC369500.044.4221936
GGCATCT359800.044.2385537
TAGGCAT383950.044.19967735
CGTATGC387000.043.90049744
ACTTAGG410600.043.65801232
CTTAGGC409500.043.61290433
CGTCTTC379500.043.5040251
CTCGTAT377950.043.45109642
Produced by FastQC (version 0.11.5)