FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6114808
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3273625.353594094859561No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG183080.29940433125618987No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC75700.12379783633435423No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC74650.12208069329404947No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC73190.11969304678086376No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT69900.11431266525457545No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA68550.11210490991704074No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC63330.10356825594523983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG42800.046.6791152
ACCGTGT49050.039.8921363
CCGTGTT52650.037.0156444
CAACCGT60000.035.1448061
ACAGACT31350.029.3118612
CGTGTTC70150.027.733685
GTTCACT72050.027.272348
TTCACTA76400.026.3137729
TGTTCAC78000.025.8131267
CAGACTC39850.024.2620963
CACAGAC43300.022.9911791
ACAACCG39650.021.4464132
GTGTTCA98500.020.171886
AGACTCA54700.018.236344
ATTAGGC16900.016.7596245
CTCAGAA56950.016.6065351
TATTAGG17450.016.1437954
CCGGTAT2651.2589699E-515.3682041
CACTAGC72650.015.16767410-11
CTTTATT68350.015.1608471
Produced by FastQC (version 0.11.5)