FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n02_3022_D3_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n02_3022_D3_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696736
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG523190.29564208902703865No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC283090.1599673521716095No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA271770.15357069235818402No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT210420.11890328250362102No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC207860.11745668805818203No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT204750.11569930183735577No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA188440.10648291300723478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT113800.046.423251
AACCGTG116550.045.2450642
ACCGTGT149350.035.3398863
CCGTGTT167650.031.5383954
ACAACCG52450.030.9146422
CGTGTTC184550.028.7521655
TTCACTA233350.022.8802729
TGTTCAC239550.022.4646647
GTTCACT242400.022.1811498
GTGTTCA254000.021.3346846
AACTCAC177900.017.5157285
ACTCACC179850.017.2996856
ATTAGGC57050.016.2293535
CACAACC108700.015.8689431
AGACTCA127100.015.7526874
GAACTCA225000.014.5802024
AGAACTC229150.014.0905363
TATTAGG67000.013.9594694
ACAGACT132750.013.7368722
CACTAGC220550.013.11634510-11
Produced by FastQC (version 0.11.5)