FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n02_3021_D5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n02_3021_D5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292936
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG482890.26397621464372917No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC265740.14526919024917595No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA221610.12114512399759121No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC184940.10109913466050502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT108900.045.274021
AACCGTG113350.042.873232
ACCGTGT142900.034.0404553
CCGTGTT160650.030.3671344
CGTGTTC175750.027.9185185
ACAACCG53200.025.6196732
TTCACTA219800.022.4946299
TGTTCAC228650.021.891187
GTTCACT228150.021.6713548
GTGTTCA244700.020.8392936
AACTCAC174900.018.272855
ACTCACC178600.017.3416796
GAACTCA217250.014.8622354
AGACTCA135400.014.4398234
CACTAGC199650.013.85384510-11
AGAACTC228500.013.6368653
CACAACC110300.013.4225951
ACAGACT132450.013.3420672
TCACTAG201150.012.41870610-11
CTAGCAA228900.012.15592612-13
Produced by FastQC (version 0.11.5)