FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3025_D5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3025_D5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15068708
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG658610.43707131361228846No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC438700.29113312169829025No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA311870.20696532177808474No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC283470.18811831777482182No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT265430.17614648847134073No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC237920.1578901124104336No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT232290.15415389295485718No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA208080.13808748566897705No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC202030.13407254291476084No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC191080.12680582834308024No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC185710.12324215188189988No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG184140.12220025764650826No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG179270.11896839463608956No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG177900.1180592257810026No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA170290.11300902506040995No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC160770.10669129695790773No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC160310.106386028583207No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA158780.10537067942387628No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT154990.10285553346710281No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA152400.10113673979215737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT126850.052.1871871
AACCGTG134950.048.758922
ACCGTGT171150.038.4731063
ACAACCG52500.036.8838962
CCGTGTT186950.035.1712884
CGTGTTC203200.032.4974025
TGTTCAC252050.026.5719977
TTCACTA255450.025.850359
GTGTTCA260050.025.6461146
GTTCACT262050.025.1813478
ACAGACT117300.022.2379042
CACAACC94250.021.6002221
AGACTCA125150.021.4437754
AACTCAC223000.021.3079815
ACTCACC227300.021.0082726
GAACTCA256400.019.1188744
AGAACTC258000.018.9456563
CACAGAC160100.018.0313681
CAGACTC164200.017.374473
TCACCAT297100.016.294118
Produced by FastQC (version 0.11.5)