FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3022_D3_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3022_D3_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696736
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG547160.3091869596743716No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC336460.1901254559032807No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA282090.1594022762163599No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT240370.13582730736334656No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC211580.11955877061171055No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT200970.1135633147265123No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA178820.10104688231773362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT109500.048.5649951
AACCGTG119650.044.0741962
ACCGTGT154750.034.1070983
CCGTGTT172400.030.588014
CGTGTTC186250.028.389125
ACAACCG48950.027.4610792
TGTTCAC242700.022.3282767
TTCACTA240150.022.1348049
GTTCACT250550.021.4036038
GTGTTCA258300.021.1253286
AACTCAC192950.019.584255
ACTCACC191200.019.3456136
AGACTCA135850.017.2638074
GAACTCA231850.016.8659924
AGAACTC235100.016.4329243
ACAGACT139000.015.8246942
CACAACC101500.014.7774491
ATTAGGC61100.014.6922335
TCACCAT280200.013.838268
CACAGAC183250.013.3424071
Produced by FastQC (version 0.11.5)