FastQCFastQC Report
Tue 22 May 2018
H2W37AFXY_n01_3021_D5_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2W37AFXY_n01_3021_D5_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292936
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG496660.2715037105033331No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC313240.17123549768063476No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA232810.12726770596037726No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT211680.11571679909665676No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC189190.10342243585174081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT111400.044.783251
AACCGTG116150.042.570252
ACCGTGT151650.032.8208773
ACAACCG51450.029.6898272
CCGTGTT169750.029.4873964
CGTGTTC188050.026.5428645
TTCACTA227550.021.9765859
TGTTCAC238500.021.499737
GTTCACT237850.021.242268
GTGTTCA258550.019.9778986
AACTCAC190750.018.9231455
ACTCACC191300.018.475646
AGACTCA138050.015.8992674
GAACTCA234050.015.8440054
CACAACC108600.014.9806851
AGAACTC243300.014.7973273
ACAGACT138250.014.1088832
ATTAGGC54450.013.9834325
CACTAGC210450.013.46678210-11
CTTTATT242250.012.6357961
Produced by FastQC (version 0.11.5)