FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7006939
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880545.538138693657816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT41450.014.27567612-13
CGTCTGA41050.014.19285516-17
CCAGTCA36150.013.82673626-27
CACGTCT40450.013.69339514-15
ATGCCGT47250.012.7273694
TGAACTC47850.012.57491620-21
AGAGCAC59500.012.4105029
AGATCGG78750.012.32951
AGCACAC49850.012.24942210-11
GATCGGA81900.011.9113342
GCCGTCT50750.011.7565686
CTCCAGT44650.011.56118124-25
CGGAAGA88400.011.4094335
TCGGAAG86950.011.2729474
ATCGGAA94100.010.7686413
TGCCGTC58950.09.79975
TCTGAAC61200.09.56055918-19
TATGCCG63000.09.3200433
AGTCACG32250.09.03559228-29
CAGTCAC33250.08.763844528-29
Produced by FastQC (version 0.11.5)