FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9724I-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9724I-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805808
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG27700.09.77368128-29
GTTTCGG30800.09.021214534-35
CCAGTCA39000.07.42899226-27
CGGGGGG5701.8506471E-67.083615376-77
CGTATGC24150.06.88352346-47
CACGTCT41050.06.768657714-15
AAGGGGG26200.06.61755774-75
CACACGT42550.06.585857412-13
TGCCGTC19650.06.526210350-51
CGTCTGA43900.06.437474716-17
CTCGTAT26850.06.102877644-45
TCACGTT48350.06.09057430-31
ACGTTTC48150.06.066506432-33
TTCGGAA50950.05.82633836-37
CTCCAGT51650.05.65552124-25
AGCACAC51250.05.514205510-11
CGGAATC46400.05.42522538-39
CGTTTCG27700.05.401204632-33
TGAACTC53650.05.40044520-21
GAGCACA54300.05.3358389
Produced by FastQC (version 0.11.5)