FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9724A-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9724A-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282504
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC42500.012.51667350-51
ACGTGGC48650.011.42237732-33
CGTATGC50500.010.95696146-47
TATGCCG50600.010.65376348-49
GGCCTTA51500.010.60581636-37
CTCGTAT52700.010.364367544-45
TCACGTG57700.09.87786630-31
AGTCACG59300.09.57134528-29
ATCTCGT58150.09.47466542-43
CCGTCTT59150.09.43504452-53
AAGGGGG38250.09.06562474-75
GTGGCCT65400.08.89634934-35
AGGGGGG26900.08.211205574-75
GCCGTCT40700.07.81882152-53
GTATGCC45000.07.33550448-49
CCAGTCA84250.07.018741626-27
TATCTCG46900.06.734484742-43
CACGTGG50800.06.544670632-33
CCTTATC82300.06.46357438-39
CGTGGCC52450.06.293507634-35
Produced by FastQC (version 0.11.5)