FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9723I-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9723I-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934022
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC190880.27528034955758723TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA59550.035.971459
GATCGGA58050.035.5346031
AGAGCAC66600.032.235218
TCGGAAG66900.032.0367553
ATCGGAA76750.028.1740932
CGGAAGA78900.027.46544
TCACGGC45450.026.59585430-31
AGTCACG45950.026.56486728-29
ACGGCTA48150.025.35110332-33
CACACGT49900.024.5573112-13
CGTATGC48650.024.553644-45
CGTCTGA50700.024.54445516-17
CACGGCT43900.024.18093330-31
TGCCGTC48800.024.13765148-49
CACGTCT51400.024.02538114-15
GGCTACA50400.023.74816534-35
CTCGTAT49850.023.58142942-43
GTCACGG46400.023.18516728-29
TATGCCG51500.023.0564746-47
GCCGTCT44500.023.05456448-49
Produced by FastQC (version 0.11.5)