FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9722I-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9722I-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7533432
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC141050.18723206103141304TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG132060.17529858900963066TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG58600.029.5025828-29
TGCCGTC59500.027.97868248-49
CTCGTAT62900.026.12652642-43
CGTATGC65550.025.83110844-45
TATGCCG67400.025.0163946-47
TCACGAT70800.024.58651430-31
ATCTCGT63000.024.38877340-41
AGATCTC63700.024.15804338-39
CCGTCTT71250.023.66462750-51
CCAGTCA73200.023.48849126-27
CACGTCT74400.023.0776514-15
CGTCTGA74700.023.01675816-17
CACACGT77400.022.30596712-13
GAGCACA88950.021.3052189
AAGGGGG51850.020.42953372-73
TCAGATC76900.020.38185936-37
AGCACAC85850.020.11051610-11
TGAACTC88750.019.6674520-21
GATCAGA85500.019.66497234-35
CTCCAGT90100.019.42548224-25
Produced by FastQC (version 0.11.5)