FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9722B-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9722B-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944774
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA37450.08.37053628-29
CGTATGC24750.08.34783244-45
CCAGTCA39400.08.07683826-27
CGTCTGA38550.07.9468216-17
AAGGGGG23300.07.848522772-73
TATGCCG27450.07.613329446-47
TGCCGTC24100.07.489175348-49
CACGTCT39350.07.48350514-15
TCACACT43200.07.47626730-31
CACACGT39100.07.47061612-13
AGGGGGG18450.07.465943372-73
GAGCACA51750.07.34259
ACACTTG43300.07.29443832-33
AGAGCAC56550.06.97128638
GTCACAC27600.06.969588328-29
CTCGTAT27650.06.87104742-43
CTCCAGT48100.06.66539924-25
AGCACAC46800.06.34298610-11
ATCTCGT32400.06.156903340-41
TCTGAAC54200.06.090390718-19
Produced by FastQC (version 0.11.5)