FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH9210A-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH9210A-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6892372
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA302480.4388619766895925TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT202300.29351288641994366TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC83750.035.38882450-51
TATGCCG89100.033.6904948-49
CGTATGC89250.033.63386546-47
CGATCTC74600.033.52181240-41
CCGTCTT94050.031.51305852-53
CTCGTAT89000.030.95322644-45
AAGGGGG61100.030.78634874-75
ATCTCGT85250.029.58476342-43
AACGATC89800.028.1915438-39
AGTCACG110100.027.8898428-29
TCACGTG111400.027.6072130-31
TGCTTGA112600.025.98444460-61
ACGTGAA120350.025.65282632-33
CTTCTGC120950.024.60272256-57
GAAACGA117600.024.37462236-37
CCAGTCA126850.024.1884726-27
GTCTTCT125200.023.76747954-55
GTGAAAC127200.023.74859634-35
TCTGCTT128950.022.83712658-59
CTCCAGT140450.021.82943324-25
Produced by FastQC (version 0.11.5)