FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH8584B-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH8584B-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456096
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA133970.20750930593349293TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT124860.19339861117306806TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG48500.031.28902832-33
CCGCACA46550.030.66110236-37
TGCCGTC48800.029.92895950-51
GTCCGCA50600.029.61500234-35
TATGCCG50300.029.46136948-49
CGTATGC50800.028.65693346-47
AGTCACG55800.027.53613928-29
TCACGTC58050.026.05974630-31
CTCGTAT56150.025.63042644-45
GCACATC53500.025.30195238-39
CCGTCTT56350.024.90748652-53
ATCTCGT58700.023.18192342-43
AAGGGGG37200.022.79258374-75
CCAGTCA71450.021.57125526-27
ACATCTC65550.020.9043240-41
CTCCAGT79950.019.36698724-25
CGTCTGA86050.017.93888516-17
TGCTTGA80400.017.66355360-61
CGTCCGC47350.017.2532734-35
TGAACTC91450.017.2431820-21
Produced by FastQC (version 0.11.5)