FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH8584A-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH8584A-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562142
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA67000.14686083861484364TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA20450.032.39982636-37
CCCGATC19950.027.25990738-39
ACCCGTC27100.026.3772232-33
CCGTCCC27750.026.01611334-35
CGTCCCG22050.025.95614636-37
TGCCGTC29250.024.76314250-51
TCACCCG29500.024.5536930-31
TATGCCG31450.023.18192748-49
CGTATGC31750.022.81328846-47
CGATCTC23450.022.68490440-41
AGAGCAC55400.021.8621189
GCCGTCT26750.021.39562852-53
CTCGTAT30550.020.98864244-45
CCGTCTT34700.020.73695452-53
CCCGTCC27400.020.71472234-35
CCAGTCA39450.019.50470226-27
CACCCGT30150.019.29792232-33
AAGGGGG20700.019.27585274-75
AGTCACC39150.019.22953628-29
GATCGGA62450.019.0236192
Produced by FastQC (version 0.11.5)