FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH8583B-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH8583B-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6102758
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA107640.17637926983177116TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT62110.10177365709077765TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC34950.025.82049650-51
CGTATGC38800.024.60494846-47
TATGCCG41300.023.40305148-49
AAGGGGG37850.022.2758974-75
CTCGTAT42250.021.52782644-45
ATGTCAG71700.021.4295634-35
ACATGTC71950.021.19006732-33
TCACATG71800.021.13519930-31
AGGGGGG34000.020.46775476-77
AGTCACA75150.020.2879328-29
CCAGTCA73800.020.20854426-27
ATCTCGT47300.019.98251242-43
CCGTCTT44100.019.0630752-53
GTCAGAA81100.018.65291636-37
CTCCAGT83000.018.28329524-25
GAATCTC62700.017.30919340-41
CGTCTGA90100.017.00055316-17
GCCGTCT32150.016.6199552-53
CACGTCT94800.016.30800414-15
CAGAATC86450.016.23493838-39
Produced by FastQC (version 0.11.5)