FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH8583A-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH8583A-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8146766
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA206110.2529960968561022TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT97030.11910247575541019TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC57000.028.66554850-51
CGTATGC63450.026.2754146-47
CCGTATC67450.025.84358638-39
TATGCCG65000.025.83168848-49
AGTTCCG76500.024.27638434-35
CTCGTAT66850.023.83737844-45
AAGGGGG46950.023.7749574-75
ATCTCGT69000.023.02585242-43
CCGTCTT72800.021.85684452-53
TTCCGTA86150.021.39162636-37
GTATCTC80150.020.41523740-41
GCCGTCT54850.020.04695552-53
AGTCACA95650.019.71409828-29
CCAGTCA99000.019.14278226-27
TCACAGT100750.018.83402330-31
GTATGCC62400.018.68717248-49
AGGGGGG43850.018.36090776-77
ACAGTTC104650.018.01866732-33
TATCTCG60900.018.01620942-43
ATGCCGT66300.017.193950-51
Produced by FastQC (version 0.11.5)