FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH8104B-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH8104B-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926607
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29950.018.2372850-51
CGTATGC35400.015.36247846-47
CTCGTAT40400.013.22605244-45
TATGCCG42050.013.102406548-49
CCGTCTT45150.011.93980352-53
ATCTCGT44050.011.75275442-43
AAGGGGG32250.011.70966374-75
AGGGGGG23000.011.15258976-77
GCCGTCT30250.010.83384952-53
AGTCAAC56950.010.09138334-35
CCAGTCA55400.010.0307926-27
AGTCACA57350.09.9381828-29
ATGCCGT37200.09.32049550-51
GTATGCC37700.09.07089648-49
TCACAGT63300.09.04153930-31
ACAGTCA64200.08.80381732-33
CAATCTC64050.08.37950540-41
TGCTTGA67450.08.27401560-61
TCGTATG41500.08.24030846-47
CTCCAGT69450.08.03571824-25
Produced by FastQC (version 0.11.5)