FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH7838Red1-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH7838Red1-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8870922
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33300.012.19494950-51
GAGCACA54950.011.9281199
TATGCCG36700.011.64754948-49
CGTATGC35450.011.52232946-47
AGGGGGG20450.011.4980474-75
GCCGTCT30650.011.15732750-51
GTCACAC31750.010.92036728-29
CTCGTAT40900.010.6256644-45
TATCTCG34050.010.60118940-41
GATCGGA67300.010.3808911
GTATGCC34150.010.29198446-47
AGAGCAC64250.010.2755418
CACACGT41200.09.91423812-13
TCGGAAG71900.09.8475563
CACGTCT42850.09.80958314-15
CCAGTCA45400.09.57245526-27
AAGGGGG30050.09.56363274-75
CGTCTGA44650.09.3077516-17
AGTCACA47900.09.22158428-29
ATCTCGT47250.09.19766142-43
Produced by FastQC (version 0.11.5)