FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH7797C-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH7797C-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590755
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC59640.10667611082939603TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41450.021.2113251
CGTATGC29050.021.0914244-45
GAGCACA43600.020.8076349
TATGCCG29250.020.62253446-47
CACACGT35800.020.43157412-13
CGTCTGA36800.020.06979216-17
CACGTCT36950.019.98831714-15
TGCCGTC28200.019.53768348-49
AGAGCAC47300.019.381168
AGTCACC37300.019.35508328-29
AAGGGGG26250.018.36673772-73
TCGGAAG50500.018.1608433
CCAGTCA40300.018.14997326-27
AGCACAC41600.017.75444410-11
CTCGTAT32400.016.85842542-43
ATCTCGT33200.016.30913540-41
CTCCAGT46600.016.10391624-25
CCGTCTT34900.015.78689750-51
ATCGGAA58700.015.7865062
TCTGAAC49900.015.18170118-19
Produced by FastQC (version 0.11.5)