FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH7797B-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH7797B-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9774097
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC140960.14421792621865734TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC43650.019.31422648-49
GCCGTCT39500.018.7581848-49
CGTATGC48100.017.52735944-45
AGGGGGG30400.016.79660272-73
TATGCCG50850.016.7662846-47
TCACGCC52300.016.11980830-31
AGTCACG54200.015.86143528-29
GTCACGC50250.014.98153828-29
CTCGTAT57500.014.78592342-43
GTATGCC52200.014.69485344-45
ATGCCGT51850.014.56503746-47
TATCTCG52800.014.07805138-39
CCGTCTT61400.013.4599750-51
AAGGGGG37550.013.40856972-73
CACGCCA56800.013.29572330-31
TCGTATG58050.013.21397642-43
ATCTCGT64650.013.07719740-41
TCTCGTA67600.011.27694740-41
ACGCCAA79650.011.18089332-33
CCAGTCA79500.011.05271226-27
Produced by FastQC (version 0.11.5)