FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH7602Red1-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH7602Red1-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610836
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT18900.011.05725914-15
CACACGT18750.010.51243812-13
TGCCGTC17900.010.48095150-51
GAGCACA26300.010.2937999
GATCGGA24600.010.2382521
CGTCTGA19350.010.06374316-17
TATGCCG19700.09.88494548-49
AGAGCAC30100.09.3098388
GCACACG15100.08.96446810-11
TCGGAAG32400.08.9454883
AGCACAC22300.08.7324410-11
CGTATGC21800.08.71485446-47
GCCGTCT16100.08.40766950-51
CCAGTCA22650.08.38780626-27
ATCGGAA39700.08.1388822
AGGGGGG10650.08.0286374-75
ACACGTC17350.07.93880512-13
ACGTCTG16850.07.892503314-15
CTCGTAT23650.07.832313544-45
AGTCACA26250.07.78028928-29
Produced by FastQC (version 0.11.5)