FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH7602I-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH7602I-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192089
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT255190.3115078461671986TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA97300.028.849499
AGTCACG59350.028.20992328-29
ACGAGTG59450.027.96273632-33
TGCCGTC58450.027.91277750-51
TCACGAG62650.026.76190830-31
AGAGCAC106250.026.598648
CGTATGC64850.025.67061246-47
TATGCCG64350.025.53808848-49
CACGAGT55900.025.15031230-31
CTCGTAT63800.025.05085644-45
AGGGGGG40700.024.50899574-75
GCCGTCT57150.023.97675350-51
GATCGGA116650.023.6697431
CGAGTGG59700.023.62901332-33
GTCACGA60950.023.06648828-29
GAGTGGA73450.022.98839434-35
TCGGAAG124500.022.9383493
GTATGCC60800.022.88875846-47
ATCTCGT70350.022.78598642-43
TATCTCG59800.022.63611240-41
Produced by FastQC (version 0.11.5)