FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_AH6978I-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_AH6978I-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5949091
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG158830.2669819641353612TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC38250.028.31115248-49
AGAGCAC72550.027.8890449
TATGCCG41900.026.0149446-47
CGTATGC43000.025.8464944-45
GCCGTCT37300.025.5941950-51
ACTGACC43650.025.3527932-33
GATCGGA81900.024.7735352
AGATCGG83800.024.3268851
GTATGCC40950.024.24077446-47
TCGGAAG86000.023.7576274
CTCGTAT42400.023.74784342-43
CCGTCTT46300.022.97847450-51
CCAGTCA48950.022.80219326-27
ATGCCGT43350.022.46047448-49
CGGAAGA91900.022.0255685
ATCGGAA95200.021.6114523
CGTCTGA52700.021.31465516-17
CAGTCAC48100.020.93382328-29
AGTCACT54000.020.80152528-29
TCGTATG46500.020.78574644-45
Produced by FastQC (version 0.11.5)