FastQCFastQC Report
Wed 30 May 2018
H2KH2AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93595130
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10821031.1561531032650951No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1894420.2024058303033502No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1430770.15286799644383206No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1403710.14997682037516266No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1375380.14694995348582773No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1274340.13615452000547465No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1270430.13573676322689013No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1224180.13079526680501433No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG1085140.11593979302128218No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1058070.11304754852095403No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1012240.10815092622874715No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1007650.10766051609736532No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC995960.10641151948824687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA4193900.065.121581
TTCCGAT4229300.064.4802862
CCGATCT4347050.062.393114
CGATCTG2118850.056.8148965
TCCGATC5575000.049.1035843
CGATCTA841700.045.7028855
CGATCTC1827550.036.4442445
TCTTCCG2518000.036.2406655
GTGCTCT2541050.036.1976621
CGATCTT1355200.035.143785
TGCTCTT2717800.033.6069342
GATCTGG1160900.032.999116
GATCTGA960400.028.0507836
GATCTAG470400.026.6019066
GCTCTTC3491650.026.3636723
TATAGGT1047150.023.8245243
ATCTGGC632200.023.1832547
CCATACT897100.023.1493532
ATACTCC898300.023.020824
CATACTC927550.022.8342933
Produced by FastQC (version 0.11.5)