FastQCFastQC Report
Wed 30 May 2018
H2KH2AFXY_n01_p2H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGCAGCCAGAAGCACAATAATGAAATTTTTCAGCATTCCTCTG13.8461538461538463No Hit
CTTCCCCACTGGGATTTTCCTTTTCTTCTCCTTTTTTCTCTACCCATCGA13.8461538461538463No Hit
TTGTTATATCATCAAGAAACGTGGAAAACAAAAGAAGAAGAAAAAAACTC13.8461538461538463No Hit
ATCACATCCGAAGACTCCGACAAAAGTTAAAAGGAAGGTGACTACGTACC13.8461538461538463No Hit
GCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGTCGACGTCTCC13.8461538461538463No Hit
GAGATAGCTCGATCGCTTCAATCTCATGCTCTGTTTCCTCGTTGACGTTG13.8461538461538463No Hit
GCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAATTAACATCGCCGGA13.8461538461538463No Hit
GGTTATGGAAGAAATACACAGATATGTATCCAAATGATGACCTTGTTTAC13.8461538461538463No Hit
CAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTG13.8461538461538463No Hit
GGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACCAGACTCG13.8461538461538463No Hit
GTACAAATACCTAGAATCTATATTATTTATAAGGGACCAGAAATACCTTG13.8461538461538463No Hit
TGATGAAGATGATTCAGATGGTATTGATGTTGGTGAGTCTGAGGAAGACG13.8461538461538463No Hit
AAAAAAAAGAGATAAAAAATACATGTCCACTAATACAAGCAGGATGAATA13.8461538461538463No Hit
GAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCGAAGC13.8461538461538463No Hit
CGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCC13.8461538461538463No Hit
GTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAA13.8461538461538463No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13.8461538461538463No Hit
GCTCTACACAACGAAAAACTCGCTACCTCACCCTCAGAGCAGAGTTACAC13.8461538461538463No Hit
CGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACA13.8461538461538463No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG13.8461538461538463No Hit
GTCTAAACCCAGCTCACGTTCCCTAATGGTGGGTGAACAATCCAACACTT13.8461538461538463No Hit
TTATATAAGCAGTGATTTCAAATCCCGAGGTTAATCGTACTCTGTCAACT13.8461538461538463No Hit
GGCCTACCATGGTGGTAACTGGTGACGGAGAATTAGGGTTAGATTCCGGA13.8461538461538463No Hit
GAGTTGAACCTTTGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGTT13.8461538461538463No Hit
TCTCGGTGAAGATGATGCTTAATCAATCAATCCTCCACAGAGAAACATAA13.8461538461538463No Hit
GCTTGTTGCCAAACCCCTTCGCAATCAAAGTAGGCCGCGACTCTTGTATT13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)