FastQCFastQC Report
Wed 30 May 2018
H2KH2AFXY_n01_p2D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCACCTCGCTTAAGATAGGGACAATGAGAGGGTTCATCTGCGCAATG12.272727272727273No Hit
CCTATAAGCAACATCATATGATCCCTGGTCGGCATCGTATGGTTGAGACT12.272727272727273No Hit
TTTAAGAACAATGCATTTGACTGAATTTCGCTTGTTGTTCATCGGATTGG12.272727272727273No Hit
AGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGAT12.272727272727273No Hit
CTTCCGATCTGACTAGGGGTGCTCTTCCGATCTATATGGGGAACCAAACA12.272727272727273No Hit
GGTATGTAGTCTATGCCAAGTCTCATCGTTTTATCATTGGAAACTTGATA12.272727272727273No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT12.272727272727273No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC12.272727272727273No Hit
TTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGA12.272727272727273No Hit
TTGCGGTATAGGCCATTCATCATTATTCGTCGTCTCGTGTGTATTCCGAA12.272727272727273No Hit
CAGAGCAACAAAACCCTAGCTACATTTCTCGATATCGGAGAAAAGATCCG12.272727272727273No Hit
GGCATGGCAACTTGATTTAGCAGAGGGAAGGTCAAGGTTATGTTTTCCTT12.272727272727273No Hit
GGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCG12.272727272727273No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12.272727272727273No Hit
CTTTTCAACCAAGCGCGATGACCAATTGTGCGAATCAACGGTTCCTCTCG12.272727272727273No Hit
CTCTTCCGATCTGCGTGGGGGCTGGAGGTTTGAGTTTCCTTCAATTCTGC12.272727272727273No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTAATAAGGTGCCAG12.272727272727273No Hit
ACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCA12.272727272727273No Hit
ATTCCGGAGAGGGAGCCTGAAAAACGGCTACCACATCCAAGGAAGGCAGC12.272727272727273No Hit
GATACATGGAGCTAAACATCCGATGGCTCAAATGCACGTTAAGACTGCTC12.272727272727273No Hit
ACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGG12.272727272727273No Hit
GCAAAGGACTTTTAAATTGCTTAATGAGCTCATAAGGCACTTTTCATCCC12.272727272727273No Hit
GCGGTATGGTACTCAATTACTGCTAAAGAGATCTCGACTGTTAAGACACT12.272727272727273No Hit
ACATCATAGGATTTCGATCATATTGTGTTGTCCTTCGGGATATGAGTAAT12.272727272727273No Hit
TTTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT12.272727272727273No Hit
CATGAGTTTGACCCAGTTGGTTCAAATCTTGTTTGGGAGCAAGTCTTAAA12.272727272727273No Hit
ATCATCACCATTCACCATCATAACGATGTGTGGTCTTATATATAGGTTTT12.272727272727273No Hit
CTGGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGT12.272727272727273No Hit
CAACAACATCTGATGAAATGATGATGATGATGATGATGATGATGATGATC12.272727272727273No Hit
CCTTTAGATACACCCAGAATCACAGCGATTGTTGCTAGAATAGCAGCGAT12.272727272727273No Hit
GCTTCTCAATCCCTAAAGCTTTCCTCAAACCCTCTTCCCGCTTTTCAACA12.272727272727273No Hit
CTCCTAGTGAAGTGTTCGCCTATCCAGGTATGGAAGTATTCAATGAATAC12.272727272727273No Hit
GTGCTGAAGCCTACTGTTTCATTGACAGAGTCTGATCTCATTTTTTTGAA12.272727272727273No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC12.272727272727273No Hit
ATCGTGAACATGTCTTTTCCCACTTTCCCAAACTGCAATATGATCTTGTC12.272727272727273No Hit
GTTTTTACCTTCCTCATGAGTAGTTAATCGGCCTCTAAAATAAAAATGAA12.272727272727273No Hit
GGGGGTGCCTCAAACTCCTTTGGCCTAAACGGCCATAGTCCCTCTAAGCA12.272727272727273No Hit
GGTTTATCTGTGACTATTGTCAGAGTTGATACCTCATCCCTTTAGGCATA12.272727272727273No Hit
TTCATTATAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACGCATCAT12.272727272727273No Hit
TCTCAGGGTTAAGGGCCCCCAGTGCATTAGATTATTTGGGAAGGCCTAGT12.272727272727273No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGA12.272727272727273No Hit
GCTGACAAATGGTGTGACTTTTTGTACGTGTGACGATCACAATGAGAGTG12.272727272727273No Hit
GCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGA12.272727272727273No Hit
ACCAAACATAGAAGTATCAAAAGCTTCCAAAGCATCCTCAACCTCTCCCC12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)