FastQCFastQC Report
Wed 30 May 2018
H2KH2AFXY_n01_p2B7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2B7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAACGCCGAAGACGTCCGATTTTCAAGCTGGGCTCTTCCCGGTTCGC12.631578947368421No Hit
TTGTTATTTATTGTCACTACCTCCCAGTGTCAGGATTGGGTAATTTGCGC12.631578947368421No Hit
CTCCGGAATCGAACCCTAATTCTTAGTCAACCGTTACCACCATTGTAGGC12.631578947368421No Hit
CCTCTCCGAAAGGGTCAACATCATCATCATCTTCTTCATCAGCCGCAGCA12.631578947368421No Hit
ACATGTAACTATACAAGCATGCACTTCGTCAGACTCAAAGTAAACCTTTT12.631578947368421No Hit
CTCTTGCAGTCCTCATCGTTGGAGCTTAGAAGCCGTCGGCACAAGAAGAA12.631578947368421No Hit
ATTCATAAACCAAAAACTCACGGTTGGGTTCAACAGACATACCAACTCTT12.631578947368421No Hit
GAGTAGTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATTAG12.631578947368421No Hit
GGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGA12.631578947368421No Hit
GTTTTACTCTGCGCCGTTTGCAAAGAGGATTTCATCATCGGAGAATATGC12.631578947368421No Hit
ACGCGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCT12.631578947368421No Hit
ACCGTTGTTAAGGGATTTGGATACAACAATAAGAAGGCTCTTCTGGTTGG12.631578947368421No Hit
GGTACTAACCTTCAATGGAAGCAGCATAGGACTAGTATAATCTCCGAAAC12.631578947368421No Hit
AATAAATATGATGCTTTAGCTCTTTCACATTGCTGAACAATGAAAACTTT12.631578947368421No Hit
CTATTATGTCATGGTTATGGACATGCTTGGGCCTAGCTTATGGGATTTAT12.631578947368421No Hit
TTGCTGATATTGTCACAATCTGCAACTTGAACTTGGGATTTGCCACCGTG12.631578947368421No Hit
GAATGGAACACTATAACTATAACATATGAAGCTAAAACACTATTTTGCTC12.631578947368421No Hit
GATCTTGTCCGTTCATTCACTCAATCGTATCCTTCATGGCTTTCTCATCC12.631578947368421No Hit
CTCCAGGCGTCCTTGGCTCGGATTTAGGCCAACCGCGTGCGGTAACACAC12.631578947368421No Hit
ACAGTGAACACTGTTCTTCCTTCTCATGCTCCAAACAACTTCATTCAAGG12.631578947368421No Hit
CTAGTGCAATGTGAAAAGCCAACACTGCAATCCATAGTTTGGGGTCAGCA12.631578947368421No Hit
AACTACTGTATTAGGTCCGTAGAGACAAGTTCGAAACATACATCAGAGGT12.631578947368421No Hit
CCATCAATGACATGCAATTCACTTTCATAAATAGTCACTGGAAGATCCTT12.631578947368421No Hit
ATGGTATTCAGAATGGTGAATACCGGAATCTCTACAATCATGAAAAATAT12.631578947368421No Hit
CAGCTTACCCTCCTCAACCATACCCTCCGCAACCATCATATTACCCACAA12.631578947368421No Hit
TCCCCAATCAGATGCAGACATACTTCAGTGAACCAAGGCTGTTGATTCTT12.631578947368421No Hit
GGCCTACTAATGAAACTGAATAAAATTCATTCTGATTACGAACGCCAAAT12.631578947368421No Hit
GGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTA12.631578947368421No Hit
TCTCAAAGTATCCACCTCGCTGTCCACATTCACCCCAATATCCTTTAGAG12.631578947368421No Hit
TCCCTAAAATGAGCTCATCGCGAACAGATATCTCGTGTGGAACAAAAGGG12.631578947368421No Hit
GCTTAAACTGCTCAATGTATAAGTCTTGATTCAAAATCACAAGCATAGAG12.631578947368421No Hit
GTATATTCAACGACTAACTTCCGAGGAAACACTGTTCAATGTGTGGCATG12.631578947368421No Hit
GTCTAGGAAAGAGAAGGAAGAAGATTTCTTGGTTATCAAAGGACCTAAGC12.631578947368421No Hit
GTTTGATCAAGGCACTTATGACAAGCTTCTCACTGAAGCTCCCAAGTTCA12.631578947368421No Hit
CTTCTCCACTTCCCGCTCGTTTGCTCGACGCTTTCCCTAATTCCCCCGCG12.631578947368421No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCG12.631578947368421No Hit
CCAGAAGCGTATCGTCGACCGAAGGGACAAGCCGACCAATGCACACCAAA12.631578947368421No Hit
CATCAATGCTGATTAACTTGTCACATCTTCCAGAAGGCCACCAAAGAAAA12.631578947368421No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)