FastQCFastQC Report
Wed 30 May 2018
H2KH2AFXY_n01_p2A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p2A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAAAGCTCGCGCTCGGATCTTTAATAGTCCTAGCAGTTCCGACT11.1904761904761905No Hit
GAAGAGCACCGCACGTCGCGTGGAGTCCCGTCCGACCCCGGCGGAACTTG11.1904761904761905No Hit
TCCCATGGATAAGCCGAAACCCGAAAAGGCGAAAGTGATCGAGCAACTCT11.1904761904761905No Hit
GTGGCAATGTGTGGCTTGTTTCTTTCGTGCTTCCGAATATTCCGGCGTTA11.1904761904761905No Hit
GGAGCATACAGTGTAGAGAAATCGTTCCCTGTGACAATTAGAGGAACTCT11.1904761904761905No Hit
TCCCAACACCGAACAAAAAAAACAAATGAAAGATTGTTTAGCAAACCAGA11.1904761904761905No Hit
ATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCG11.1904761904761905No Hit
CTCTTTCTTAGGTTGAATTAGCTTGAGTTTAGCTTCGAATTTTATCTTTG11.1904761904761905No Hit
CGCTAACCTTAGGGCGAGAGTCAAAGAGCACCTTGGAGTCAGTACCCAAC11.1904761904761905No Hit
GTTACTGGCATGAGCGTTGAATCCACCTAGAGAGCCTGCAACAGCAGAGC11.1904761904761905No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG11.1904761904761905No Hit
GATTACAAAAAACAGAATTAACTTTTATTTTTATTTTAGGCTTTGGTAGG11.1904761904761905No Hit
GCTCTGGTTGCTGGCACAGAGGATGGTATCAAGGAGCCAACAGCAACATT11.1904761904761905No Hit
TCCTTCCCCTTACCAAAACCTTTTTGTACTTCCACATCAATTTTTTGATC11.1904761904761905No Hit
TGATGGGTCAGCGAGAAAATGGGAAGAGCGGCTTAAGCCATTAGGTGTAG11.1904761904761905No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG11.1904761904761905No Hit
GTCCCAGGCTCGGCTGTTGCGGCCCAACCACAGCAAGAGTACGAGTTTAG11.1904761904761905No Hit
GTCATCTTCATGACCACTACTACTCTTCTTTTTCACACCTAAAACCTTAA11.1904761904761905No Hit
CTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATC11.1904761904761905No Hit
ATCTAAAACCTCAAGCTTTTCCAGCAACGCTATAGACCGGATCGATTCAT11.1904761904761905No Hit
GTATTCCAACCATGGATCCAAAGGAGACGGCTTGCTTTTGCAAAACACAA11.1904761904761905No Hit
TGCGGGGGAATGCATTGGATGGATGACAGGGCATTGAGAAGGAAGGACGC11.1904761904761905No Hit
CTCATGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGAT11.1904761904761905No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACC11.1904761904761905No Hit
TACTCACCCAACATCCATATAAGAGCCGCCTTGGCTTTCGGTTCCTGGAT11.1904761904761905No Hit
AGTAGCGAGAGATCCATCTTTCAACGCGAAAGCAGACTCAGACGAGTGTG11.1904761904761905No Hit
TCACTGGAGCTCACGGCGGCCACCGCAGCCTCACAGACCGGAGCTTCCAA11.1904761904761905No Hit
TCATTAGGATCAGCAGGGAAACCATCACGGGCTTCGATACCATCAGCAAT11.1904761904761905No Hit
TCAATACTCTCCAACTTTCCTTGTGTGACATTCAATTCATTACTGATACA11.1904761904761905No Hit
GAGCATATGATATTCAAAGGCACGGATAGGCGTACAGTGAGAGCGTTGGA11.1904761904761905No Hit
GTAAAACTGTCCTCCATAGTACGGATCAACCCATTGGTTTCCATACTTAT11.1904761904761905No Hit
AACCAACCCAATTTGACAGTTACAACACAATACCAAAGAAAAAAACTTGT11.1904761904761905No Hit
GGTATACTCTGTTCATTACTGAAGAAAATCCCGGCATCAACCGCGGTTTT11.1904761904761905No Hit
AATCAGGAGTGGTTCAATCGTGAGTTTGGTTCTGCTGGCTGTATCAATTC11.1904761904761905No Hit
GCCTTAATGAATCAAGCGAGTTCGTCGGAGACAAGGTTGCCTATGCACTT11.1904761904761905No Hit
CCATTGGTTTTCACAACACTTAGTTAAAAAGGAAGTAAAAAAAGAGTAAC11.1904761904761905No Hit
CCTACGAACACTGGCTATCCGATCACCACTCATACGCCGAACGTTCAATT11.1904761904761905No Hit
TGTCGGTTGAAGAATAACTGCTGCTACTACTGTCCTCTGTTTGACAATTC11.1904761904761905No Hit
GAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGC11.1904761904761905No Hit
CTACTAGGGCCAGCTGAGCACATTCCATCTAAAATCGAAGTAGATGTGAG11.1904761904761905No Hit
GCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGCCTAACCCATGTGCA11.1904761904761905No Hit
ATCCGGTGTGGGTCAGACTTTCACAAAAGAAGTTGTCGAGGCCATGGCTA11.1904761904761905No Hit
CCCAAGACAGTTCAAGATTCCAGACTGGTTTTTGACCAGCCAGAAAGATT11.1904761904761905No Hit
CGGCGATACGCTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGT11.1904761904761905No Hit
GGTGAGGAACACCGAGGATTGAAATCGATTTACGATGAGATTTTCTCTTA11.1904761904761905No Hit
ACCAAAAATGGCCCACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAA11.1904761904761905No Hit
ATTTCAAGAGGACCTTTATACGGAGACTCAACTTCATTCTCTCCGGCGTC11.1904761904761905No Hit
GTTATACATGGATGAGTTATCAGCAGTACCGGGAAGCGGCGTTGGAAGGG11.1904761904761905No Hit
GTTCGCGCTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGA11.1904761904761905No Hit
GGTTGGCAGCCTTGGAATTGAATCCCTTAAATTCATTTCTCTTTCAGACC11.1904761904761905No Hit
AGGAAACTCTGGTGCAAGCCCGCAGCGATACTGACGTGCAAATCGTTACT11.1904761904761905No Hit
GGATTTACACCAAGAAACAAAATTTGAGGTGCTTACAAATTAGAAACCAA11.1904761904761905No Hit
CCTTCAGCCCATCAGGGAAGCTGGTTCAGATAGAACATGCCCTAACAGCT11.1904761904761905No Hit
AACCCAAGGGCAAGAGGTCAGGGCCAAGGAAGTAGGATCTCAGGTGAAAT11.1904761904761905No Hit
GCTTCGTAGTCTCTATCAGCGAAAAACATCTGATGAAGCCTTCGATCAAC11.1904761904761905No Hit
GCGCCGGAGGCACGACCCGACCAATTAAGACCAGGAGCGTATCGCCGACC11.1904761904761905No Hit
ATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAAT11.1904761904761905No Hit
GTGCTCTTCCGATCTCAATACGGCCAATCTCAAGACTACATAAGAGTAGG11.1904761904761905No Hit
GGGAGATTGCATCATCACAAACATTTCAACTTCACTGAGTCTAAGGAGGA11.1904761904761905No Hit
CACCAGTCATGATAGATTCCAAATAAACCACGTTCATATATGATGGGAAG11.1904761904761905No Hit
GTATTAACTATCAAGCTTGTTGAGCAGAAAGTCTCTTCCTGGCTTCCTCA11.1904761904761905No Hit
CTTATAACCAAGAGAATGAAAAGCACAACTAAACCAGAACCGAGAAAACA11.1904761904761905No Hit
GAGACGAAACTGTACATTGCAACTGATGGAAGGTCTAGACTACGTCCGCA11.1904761904761905No Hit
CAATAACCTTAGCCCAAGCAGGCCTGGCAGAGATCTTCTTAATCCACTCG11.1904761904761905No Hit
AGGTTAGTGAGTGAACTTAGAGAACTCAATGGAGAGGGAAAAAAAAAAAA11.1904761904761905No Hit
TCCCAAGGTTCAACTACGAGCTTTTTTTACCTGCAACAACTTAACTATAC11.1904761904761905No Hit
GTACCATAGTACAGCCTTTTTGTCATGTCCAGCGCTAGCAAGCATTTTTC11.1904761904761905No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG11.1904761904761905No Hit
GCATGAACACAGTGGCAAGAATGACACAGCCAGTGAGATTAGTTTGTTTA11.1904761904761905No Hit
CTCTCACCCAAGTACCTGAACAAGACCCTTCCTGAACTCGCAACGGTCGC11.1904761904761905No Hit
GAAATATGTAGAATTACAATCTCAAATATTGAAACATTAATCTAAAACTA11.1904761904761905No Hit
CGCCCGTATTTAGCCTTGGACGGAATTTACCGACCGATAGGGGCTGCATT11.1904761904761905No Hit
CCTTTATGTGTTCCTTGTTGGGTAAAAGCTAGAGGAACATTCAGCAGACA11.1904761904761905No Hit
TCTTTCAAAGTACTAAAGAACGGTTAGAAAGAGAAGATGAACAGCCTCTA11.1904761904761905No Hit
GGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGTGT11.1904761904761905No Hit
GAACGGGAGTGCTTCTTGTGCTTCTATGATTTGCATATGTCTGCTTCAAG11.1904761904761905No Hit
GGGCAAGAACGCGTCTCTCTCCTTCCAAGTGACCAAGGAAGGCAACCCCC11.1904761904761905No Hit
CGCTTCTTAGAAAGTCACATTACATTTTTAAGATTATATATGCCAAAAGC11.1904761904761905No Hit
GTATAATATTTGGATCACTAGCAGTCACTTAGGGTGGCATTGTCTTCAAC11.1904761904761905No Hit
ATATATACGTACAAAATGCAAATAAGAAAATCAGTGCTGTTTTGGCGGAC11.1904761904761905No Hit
GGATGATGGAAAAACCAGCAAGCCCCTCTTCTTTCAGTCGAGTTACTTCG11.1904761904761905No Hit
GAGGACGACGATCGATGGATCCTCGGATGTGGCACAAAGTCGCCGCTATT11.1904761904761905No Hit
CCACCCGTTCCTCTCCCTGTTGGATCACCCGTTTTTGCTATCAAATCCTT11.1904761904761905No Hit
CACCAAAGGCGGACCGGCCGACCCATCCCAAGGTTCAACTACGAGCTTTT11.1904761904761905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG50.0145.03
CGTAGTC50.0145.05
TAGTCTC50.0145.07
GACAATC50.0145.0145
GTCTCTA50.0145.09
CTTCGTA50.0145.02
TCGTAGT50.0145.04
AGTCTCT50.0145.08
GCTTCGT50.0145.01
GTAGTCT50.0145.06
Produced by FastQC (version 0.11.5)