FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20973239
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333661315.908906583289307No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2450501.1683936849239165Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512340.2442827261921728No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214300.10217782765933293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT96050.063.2440419
GCACACG921550.062.3279818
AGAGCAC1126050.061.8433415
AGCACAC945850.061.500817
GAGCACA1058600.061.29211416
ACGTCAG441400.060.98680522
CACACGT744050.060.9824219
CGCGCGT110450.059.4022419
ACACGTC543350.058.9665620
CACGTAG211150.058.78077721
CACGTCA457700.058.2252321
CACACGA182150.058.05405419
CGTCAGA489850.057.97780623
ACACGAG182650.057.60941720
TTAGCTA224700.057.46330319
ACACGTA212400.057.2670720
AAGAGCA1303500.056.88987714
GCGCGCG193850.056.82376518
GCTAAAC183050.056.2973622
ACGCGTC84950.055.89798420
Produced by FastQC (version 0.11.5)