FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds774-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216178
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3816593.124209552283865No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1779541.4567076543907596Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA70400.050.6088519
AAAGGGG253200.049.79993466
ACACGAG71950.049.47015420
CACGAGA75000.047.4095721
CACGTAG61400.046.79595621
GAGCACA272850.046.71618316
AGAGCAC280000.046.6478215
GCACACG205950.045.15242418
AAGGGGG284650.044.48213267
AGCACAC224200.044.1307717
ACACGTA64600.043.8839420
AGGGGGG314000.041.01870768
AAAAGGG313100.040.4849565
AAGAGCA406350.038.1894314
CACACGT152100.037.2306519
CACGCGT7400.036.4175219
ACGAGAT101650.035.15215322
GTGACTG52950.035.1172031
GCACAAG71350.035.0722318
ACGTCAG67050.034.9189322
Produced by FastQC (version 0.11.5)