FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds774-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040838
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4906383.7623195687271016Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3749462.8751679915048403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG250050.062.36940818
GAGCACA395550.062.0871616
AGAGCAC405350.061.71764815
AGCACAC278500.061.20079817
ACACGAG110850.059.67390420
CACACGA111400.059.3462719
CACACGT153700.057.47277519
AAAGGGG620600.057.3318766
CACGTAG86300.056.7745821
CACGAGA116900.056.04385821
ACACGTA88050.055.45020720
AAGGGGG645600.055.0521467
AAGAGCA592250.052.83694514
AAAAGGG697400.051.17906665
GCACAAG132950.051.1490818
AGGGGGG706500.050.65981768
AGCACAA141950.048.07868217
ACACGTC80650.048.0398420
ACGTCAG71900.047.26362222
GAGCAAG114700.046.7157616
Produced by FastQC (version 0.11.5)