FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds774-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946344
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC128564810.76185316612346Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3404802.850076977525509No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT249030.20845708109527064Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC201900.1690056807337877Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC157110.13151303863340952Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC149830.12541912404330563Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC123120.10306081927659208Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA745900.064.42293516
CACACGA213950.064.3042219
AGAGCAC758700.064.18918615
ACACGAG215200.063.94777720
GCACACG472000.063.44236418
AGCACAC519350.063.25178517
CACGTAG158250.062.67621
ACACGTA158400.062.50747720
CACGAGA222900.061.48621421
AAGAGCA999850.060.8051714
GCACAAG233100.059.7273318
CACACGT266650.059.70670319
AAAGGGG1540400.059.12222766
AAGGGGG1541250.059.02831367
AGCACAA238050.058.456217
AGGGGGG1572750.057.9616868
GAGCAAG186800.057.74392716
ACACTCT118000.056.085711
GATCGAG72800.055.821378
AAAAGGG1637500.055.75343365
Produced by FastQC (version 0.11.5)