FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds395-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564164
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3469913.62803272716779Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2535252.6507805595972633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA85500.056.61179719
ACACGAG87100.055.53196320
AAGGGGG446700.055.16980467
AGAGCAC315000.055.16506215
AAAGGGG449300.054.89699666
GAGCACA308600.054.66493216
CACGAGA89700.053.25570321
GCACACG209000.052.91712618
CACGTAG70350.052.0347521
AGGGGGG477550.051.8443768
AGCACAC233450.051.46803717
ACACGTA71400.050.68453620
AAAAGGG493200.050.24500765
AAGAGCA429100.049.70491814
GCACAAG90450.047.63266818
CACACGT137250.044.39533219
ACGAGAT110000.043.71394322
AGCACAA99800.043.1703117
AAAAAGG584700.042.60582464
GAGCAAG84900.040.97664316
Produced by FastQC (version 0.11.5)