FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds395-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9445783
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3935844.166769446217429Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3235053.424861655195763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA287350.060.7889216
AGAGCAC293350.060.71510315
GCACACG186300.060.5095118
AGCACAC206350.059.12466417
ACACGAG81750.057.58236720
CACACGA82600.057.03036519
AAAGGGG493550.056.68218666
AAGGGGG496800.056.28319567
CACACGT116350.055.52729419
AAGAGCA404100.054.42490814
AGGGGGG521550.053.8464268
CACGAGA88450.053.53512621
CACGTAG64900.053.44017421
ACACGTA64850.052.72778720
AAAAGGG546950.051.2444265
GCACAAG96100.050.33040618
AGCACAA105450.046.1000517
ACACGTC64650.046.06976320
ACGTCAG56250.045.854722
ACGAGAT106500.044.1989122
Produced by FastQC (version 0.11.5)