FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds395-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342631
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3651483.2192530992148116Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1034450.9120018098093821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA402850.056.9749816
CACACGA118500.056.88426219
AGAGCAC413450.056.6403715
ACACGAG120600.055.45891620
GCACACG265200.055.25702718
AGCACAC295100.054.23629417
AAGGGGG488800.053.1459267
CACGAGA124400.053.11610821
AGGGGGG498850.053.02510568
CACGTAG95350.052.7816321
AAAGGGG494500.052.5191666
AAGAGCA567050.052.2966514
ACACGTA98750.050.50516520
GCACAAG127700.048.89472218
CACACGT166150.046.91093419
AAAAGGG555350.046.9040765
GAGCAAG119200.045.65730316
AGCACAA137250.045.4925817
ACGAGAT146950.044.7985422
AGAGAGA209300.042.94215415
Produced by FastQC (version 0.11.5)