FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_ds395-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds395-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676523
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5285244.950338232774847Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3511233.288739227180984No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT107840.10100666668352608Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC335950.061.44332515
GAGCACA331950.061.19265416
GCACACG208350.061.00942618
AGCACAC231800.059.65381217
ACACGAG92550.059.44545420
CACACGA93250.059.11181319
AAAGGGG646700.058.0020466
AAGGGGG649200.057.79485367
CACGTAG74050.056.6200521
CACACGT128500.055.01622419
AGGGGGG685950.054.967968
CACGAGA100000.054.9462621
AAGAGCA474850.054.61429614
ACACGTA76600.054.4615620
AAAAGGG708700.053.1058165
GCACAAG112550.052.14734318
AGCACAA122700.047.86212517
GAGCAAG101600.046.19337516
ACGTCAG53350.045.98562222
ACGAGAT123800.044.29827522
Produced by FastQC (version 0.11.5)