FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958854
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1478862.125148767311399No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC282750.406316902179583Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG44150.041.46018218
AGAGCAC68100.039.52176715
GAGCACA65900.039.30126616
CACACGA18050.038.97408719
CACGTAG18650.037.15590721
ACACGAG18650.036.97010820
AGCACAC55850.036.03337517
ACACGTA19700.035.5326120
CACGAGA20550.033.89087321
AAAGGGG59950.033.50800766
CACACGT35050.031.25456619
AAGAGCA113250.028.43195714
CCGGGTC5250.026.6780741
CCGTATC183250.025.57352655
AAGGGGG80700.025.3259267
CGCCGTA190400.024.72346553
CGTATCA191100.024.66953356
GCCGTAT194250.024.26948554
GTATCAT197200.023.92417557
TCGCCGT199000.023.44396652
Produced by FastQC (version 0.11.5)