FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983656
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1008652.0239157758882236No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC193350.3879681904208477Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18250.035.09418
AAAGGGG45750.034.19248266
AGAGCAC29400.032.73633615
CACACGA7950.031.6964119
GAGCACA29200.031.52227816
AGCACAC24950.028.05460517
AAGGGGG56250.027.80988567
ACACGAG9300.026.34319720
GGGGGAT12950.026.229021
CACGAGA9450.025.55315621
CGGGGTG6150.024.4834941
GGGGAAC10300.024.4752662
CACACGT15600.024.22946519
CGCCGTA108750.023.9447453
CCGTATC108150.023.85104855
GCCGTAT111850.023.34368154
AAAAGGG68250.022.8689665
CGTATCA114800.022.68307356
TCGCCGT113950.022.57561152
GGGGTTA14200.022.1914792
Produced by FastQC (version 0.11.5)