FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7138858
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311511.8371425793873475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC160140.22432159317358602Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29050.036.6255618
AGAGCAC44800.036.2488115
GAGCACA43900.036.0355216
AGCACAC34950.033.146517
CACACGA13700.029.8891119
ACACGAG13800.029.67314720
AAAGGGG44600.029.03268266
CACGTAG13850.025.26922221
CACACGT25450.025.02829719
CACGAGA16850.024.50889621
CCGTATC109600.022.95985255
AAGGGGG57200.022.88210367
ACACGTA14950.022.70844520
CGCCGTA111350.022.5675853
CCGGGTG5650.022.311561
GCCGTAT112950.022.3098754
CCGGGTC5450.021.8453161
CGGGGAC7700.021.8286291
AAGAGCA94300.021.4892214
TAGATCG79300.021.319476
Produced by FastQC (version 0.11.5)