FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n02_163A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_163A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498983
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1545512.378079770327142No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC414410.6376536144193637Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA24750.044.6866219
ACACGAG24250.044.59837320
GCACACG70900.043.3431618
GAGCACA93300.043.1780116
AGAGCAC95500.043.1725315
AGCACAC79100.042.1246317
AAAGGGG78550.039.74057466
CACGTAG22900.039.4299721
CACGAGA27550.039.380621
AAGGGGG84050.037.0567967
ACACGTA24750.035.77812620
AAGAGCA135650.035.19331714
CACACGT53900.034.80519
ACGTCAG25450.032.17883322
AGGGGGG99900.031.95029868
GTGACTG26900.030.7224581
TCGCCGG8100.030.24558370
CGGGGAC5300.029.0718771
ACGAGAT38650.027.70857422
TGACTGG29100.027.673512
Produced by FastQC (version 0.11.5)