FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20973239
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1803210.8597670583928405TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG1628630.7765276503071367TruSeq Adapter, Index 15 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412440.1966505984125771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG89750.044.3194661
ATGCCGG52350.043.05556570
TGACTGG96450.040.6812742
GACTGGA102300.038.4594963
AAAAAGG431750.037.87307770
GTTCAGA953350.037.4993741
TCGCGTA148850.033.64743468
AGTTCAG415000.033.4369471
GCGTATG180700.033.0624870
GGAGTTC110950.032.7420857
CGCGTAT167700.032.5575469
TCTCGTT195800.031.76397570
TCGTATG2803050.031.38773370
CTGGAGT123050.030.8930745
GCTTGAA567500.029.5983760
CTCGTAT2870450.029.52531669
TGCTTGA561850.029.48485659
CATCTCG328100.029.21776266
ATCGCGT157750.029.06451267
TCTCGTG104150.028.96730470
Produced by FastQC (version 0.11.5)