FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds774-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216178
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1686401.3804644955238865TruSeq Adapter, Index 12 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG305350.040.86385770
CTGCTTG350600.036.7287658
TGCTTGA352500.036.5905259
ATGCCGG26550.036.12133470
GCTTGAA371250.035.01592660
GACGTGT139000.033.7760661
AAAAAAG397950.031.82995669
CTTGAAA412300.031.79279161
TCTGCTT410650.031.50275657
CCGTCTT419850.030.80297751
CGTCTTC431800.029.99914252
CTTCTGC433750.029.97847655
GTCTTCT435650.029.73427253
TGAAAAA452400.029.21433463
TGCCGTC442500.029.18685349
TTGAAAA457650.028.76447962
TTCTGCT451300.028.69622656
GCCGTCT450100.028.6860250
TCTTCTG458150.028.28257254
GAAAAAA488250.027.32005164
Produced by FastQC (version 0.11.5)