FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds774-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040838
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4200293.2208743027096878TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG212140.16267359505577786TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG629950.054.0150270
GACGTGT111150.045.23521
AAAAAAG772650.044.63244669
CTTGAAA886850.036.9370861
TGAAAAA966200.036.6528463
GCTTGAA885700.036.59776360
TTGAAAA901650.036.5675862
TGCTTGA863200.036.1772759
CTGCTTG872250.035.07145358
GAAAAAA1020600.035.00082864
TCTGCTT956050.032.392557
CTTCTGC972750.031.42944355
TTCTGCT945950.031.29520856
GTCTTCT973100.030.3925853
ATGCCGG147100.030.312570
CGTCTTC1004250.030.30024152
TCTTCTG991550.030.18050454
ACGTGTG192400.029.790321
CCGTCTT1047650.029.6430351
GCCGTCT1066800.028.7959550
Produced by FastQC (version 0.11.5)