FastQCFastQC Report
Sat 26 May 2018
H2JY7AFXY_n01_ds774-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n01_ds774-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946344
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG127806310.698360937873545TruSeq Adapter, Index 9 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA253170.21192257648030227TruSeq Adapter, Index 9 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG205330.17187685203104816TruSeq Adapter, Index 9 (100% over 39bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC164660.13783296379210241TruSeq Adapter, Index 9 (100% over 46bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT158330.1325342715729599TruSeq Adapter, Index 9 (100% over 48bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC153750.12870046266874619TruSeq Adapter, Index 9 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG124950.10459266868591763TruSeq Adapter, Index 9 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC122470.10251671975961851TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1448650.062.40295870
ATGCCGG54350.058.1521970
AAAAAAG1593400.057.13823369
GACGTGT148150.049.2262081
TGAAAAA2207850.043.48061863
TTGAAAA2216250.043.23581362
GAAAAAA2234150.043.1033264
CTTGAAA2294300.041.702661
GCTTGAA2520050.037.88780260
AAAAGGG124950.036.83556770
TGCTTGA2612300.036.47883259
ACGTGTG312850.036.136051
CTGCTTG2707500.035.1622758
TCTGCTT2781250.034.26385557
GAATCGG24950.034.0876662
TTCTGCT2821250.033.76799456
CTTCTGC2842450.033.57851855
TCTTCTG2903850.032.84696654
GTCTTCT2941450.032.4402853
CGTCTTC3143350.030.35995552
Produced by FastQC (version 0.11.5)